Gapdh antibody dilution
WebJan 6, 2024 · GAPDH Antibody (H-12) is an IgG2a κ mouse monoclonal GAPDH antibody that detects m, r, and h GAPDH by WB, IP, IF, IHC(P) and ELISA. 245 citations ... Rated 5 out of 5 by Angelika from very good antibody works on WB in dilution 1:500 in 5%BSA on mouse brain tissue. Date published: 2024-02-15. Rated 3 out of ... WebThe membrane was probed with a GAPDH polyclonal antibody (Product # PA1-987) at a dilution of 1:3000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room …
Gapdh antibody dilution
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WebThe GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13.3 and encodes a tetramer containing identical chains. 1] The enzyme is commonly known as glycolytic enzyme and contains a binding site for NAD + (nicotinamide adenine diphosphate) and glyceraldehyde-3-phosphate. GAPDH contributes to 10-20% … WebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产品名称. 包装. AG0122. GAPDH Rabbit Monoclonal Antibody. 50µl. AZ050. Western一抗稀释液.
WebAnti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is popular affinity purified polyclonal antibody. This antibody is supported by peer reviewed publications and … WebMar 31, 2024 · The antibody dilutions were 800-fold in most cases except for GAPDH (5000-fold dilution), B-ACT and ALB (2000-fold dilution). Washing 5 times with T-TBS (200 µL/well). Incubation with secondary antibody 4000-fold diluted with blocker for 1 h at 37 °C with 400 rpm in the orbital shaker (100 µL/well).
WebApr 1, 2016 · Brian Gregory George Oliver. Western blot analysis of A: PDE3B (n = 4 non-asthmatic and n = 4 asthmatic) and B: PDE5A (n = 4 non-asthmatic and n = 4 asthmatic) expression in non-asthmatic and ...
WebAll lanes : Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) at 1/50000 dilution Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, …
WebApr 8, 2024 · The end-point dilution, where the OD450 score was more than 2-fold of the OD450 of the background control wells, was determined as the titer of anti-CVA10 IgG. 2.9. Virus Neutralization Assay. ... 48, and 72 h.p.i. by anti-CVA6/CVA10 and GAPDH antibodies, respectively. The symbol **** was used to indicate p < 0.0001, respectively. … bbc alan sugarWebRecommended dilution: WB 1:10000 - 1:760000; IHC 1:100 - 1:500; IF/ICC 1:50 - 1:200; Antibody Dilute Procedure: Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted … davor erceg biografijaWebMonoclonal Antibody for studying GAPDH. Cited in 5519 publications. Validated for WB, WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, GAPDH (D16H11) XP® Rabbit … davor ebner biografijaWeb11 rows · Anti-GAPDH, Cat. No. AB2302, is a chicken polyclonal antibody that detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is tested for use in Western … bbc albanian migrantsWebA specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing … davor erceg dubrovnikWebSee all GAPDH antibodies. Applications Immunofluorescence analysis of GAPDH (green) showing staining in the cytoplasm of HeLa cells (right), compared with a negative control … davor dujmović deathWebAll lanes : Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915) at 1/10000 dilution ... (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor ® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary ... davor dujmović todesursache