2024 Germacrene synthase

Germacrene synthase

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August undefined, 2024

WebDec 16, 2008 · Sesquiterpene synthase that catalyzes the formation of germacrene A. Can use farnesyl diphosphate as substrate, but not geranyl diphosphate or geranylgeranyl diphosphate. Beta-elemene, the initially measured product in the assay, is derived nonenzymatically from germacrene A. 1 publication WebApr 30, 2024 · As expected, purified geosmin synthase yielded the sesquiterpenoids geosmin, germacradienol and germacrene D in the assay mixture upon incubation with FPP (S2 Fig and S3 Fig), whereas the monoterpene limonene was formed when GPP was added to limonene synthase containing crude extracts (S4 Fig and S5 Fig).

ENZYME - 4.2.3.22 germacradienol synthase - Expasy

WebIn particular, we identified a typical germacrene D synthase gene cluster within the expanded TPS gene family. The entire biosynthetic pathways of germacrenes, (+)-borneol, and (+)-camphor were elucidated in A. argyi. In addition, partial deletion of the amorpha-4,11-diene synthase (ADS) gene and loss of function of ADS homologs may have ... WebMay 22, 2024 · Germacrene A synthase (GAS) catalyses a 1,10-cyclisation of FPP to B, followed by deprotonation to 1 (Scheme 3). Both enantiomers of 1 are accessible through this reaction, depending on whether C10 of … how old based on date

Germacrene C synthase from Lycopersicon esculentum cv.

WebApr 1, 1999 · Formation of (-)-germacrene D involves a stereospecific 1,3-hydride shift of H-1si of farnesyl diphosphate. Both products are formed from a common intermediate. The enzyme mediates a key step in the biosynthesis of geosmin (see EC 4.1.99.16), a widely occurring metabolite of many streptomycetes, bacteria and fungi. WebJul 28, 2012 · A putative gene encoding germacrene D synthase (GenBank Accession No. JQ247185) from tea plant (Camellia sinensis) was isolated by rapid amplification of … WebJul 16, 2024 · The genes underlying STL biosynthesis have been partially characterized. Genes encoding the enzyme catalysing the first step of the STL production, germacrene … mercedes maybach s680 precio

Identification of the sesquiterpene synthase AcTPS1 and …

germacrene D synthase - Wikipedia

WebMar 3, 1998 · The germacrene synthase activity expressed from clone pLE11.3 exceeded the limonene synthase activity by a factor of 10, when measured in the same enzyme … WebSep 20, 2012 · Highlights This manuscript is firstly characterized for germacrene A synthase gene in Citrus species. The gene identification of mono- or sesqui-terpene … mercedes maybach s650 sedan/cabrioletWebJul 12, 2016 · Analogously, germacrene C synthase from Solanum lycopersicum clone pLE11.3 was also shown to accept more than one substrate, producing germacrene C (64%), germacrene A (18%), germacrene B (11%), and germacrene D (7%) with FDP, and limonene with GDP (Colby et al., 1998). TABLE 1. how old barney the dinosaur

"Web'Germacrene-A synthase' published in 'Class 4–6 Lyases, Isomerases, Ligases' Keywords. Escherichia Coli; Molecular Weight; Catalytic Activity; Functional Analysis " - Germacrene synthase

Germacrene synthase

Isolation and Characterization of Two Germacrene A Synthase …

WebSep 20, 2012 · Highlights This manuscript is firstly characterized for germacrene A synthase gene in Citrus species. The gene identification of mono- or sesqui-terpene could not be estimated from nucleotide sequence and enzymatic characterization is indispensable. The information of transcriptional data and gene function is valuable to consider bio … WebMar 3, 2015 · Germacrene A synthase is a two-domain, α−β-terpenoid synthase with the active center in α-domain (C-terminus, 234–558 AA) exhibiting class I terpene synthase activity (Christianson, 2008; Rajabi et al., 2013). β-domain in N-terminus (32–245 AA) has lost the catalytic activity in mono- and sesquiterpene synthases, and seems to play a ...

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WebMar 1, 2024 · Isolation and characterization of two germacrene A synthase cDNA clones from chicory. Plant Physiol., 129 (1) (2002), pp. 134-144. CrossRef View in Scopus Google Scholar. Cai et al., 2024. P. Cai, X. Wu, J. Deng, et al. Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia ... WebAug 11, 2024 · Germacrene A (GA) is a key intermediate for the synthesis of medicinal active compounds, especially for β-elemene, which is a broad-spectrum anticancer drug. …

WebDespite their importance, only four terpene synthases have been identified from the Zingiberaceae family: (+)-germacrene D synthase and (S)-β-bisabolene synthase from ginger rhizome, and α-humulene synthase and β-eudesmol synthase from shampoo ginger (Zingiber zerumbet) rhizome. We report the identification of 25 mono- and 18 … WebMay 18, 2024 · Germacrene D was derived from the linear C15 precursor farnesyl diphosphate (FPP) through highly complex reactions catalyzed by germacrene D …

WebGermacrene A is next oxygenated by cytochrome P450 enzymes germacrene A oxidase (GAO) and costunolide synthase (COS) to form costunolide (Liu et al., 2011). Another key step to convert costunolide to the guaianolide precursor kauniolide in chicory was recently characterized, and it involves the action of three kauniolide synthases (KLS ...

WebJul 5, 2004 · --germacrene D synthase: BLAST Add. Sequence: Expand table. Expression. Tissue specificity. Expressed in flowers. Detected in stems, young leaves and tendrils. Developmental stage. Expressed in flower buds and detected in open pre-anthesis flowers, flowers after anthesis and in early stages of fruit onset.

• Adio, A. M. (2009). "Germacrenes A–E and related compounds: thermal, photochemical and acid induced transannular cyclizations". Tetrahedron. 65 (8): 1533–1552. doi:10.1016/j.tet.2008.11.050. • Deguerry, F.; Pastore, L.; Wu, S.; Clark, A.; Chappell, J.; Schalk, M. (2006-10-15). "The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases". Archives of Biochemistry and Biophysics. 454 (2): 123–136. doi:10.1… how old based on birthday calculatorWeb(+)-Germacrene D synthase (EC 4.2.3.77) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase ((+)-germacrene-D-forming). This enzyme catalyses the following chemical reaction mercedes maybach s680 for saleWebGermacrene D Receptor Neurons. One type of receptor neurons, tuned to the sesquiterpene germacrene D, frequently occurred in recordings from heliothine moths, … mercedes maybach s680 cenaWebFeb 3, 2024 · EC 4.2.3.-. Acting on phosphates. Date. Modify. 2024-04-02. While germacrene A is an enzyme-bound intermediate in the biosynthesis of a number of phytoalexins, e.g. EC 4.2.3.9 from some species and EC 4.2.3.21, it is the sole sesquiterpenoid product formed in chicory. ENZYME. how old based off birthdayWebDec 15, 2004 · Two new sesquiterpene synthase cDNAs, designated Sc11 and Sc19, have been isolated from goldenrod and functional expression in Escherichia coli identified Sc11 as (+)-germacrene D synthase and Sc19 as (-)-germacrene D synthase. Thus, the enantiomers of germacrene D are the products of separate, but closely related (85% … how old bea alonzoWebMay 1, 2002 · de Kraker et al. (1998, 2001, 2002) showed that the sesquiterpene lactones in chicory and probably also in a large number of other plant species originate from a … mercedes maybach sedan 2020WebMay 1, 2002 · de Kraker et al. (1998, 2001, 2002) showed that the sesquiterpene lactones in chicory and probably also in a large number of other plant species originate from a common germacrane precursor, (+)-germacrene A.The biosynthesis of this sesquiterpene olefin from the ubiquitous sesquiterpene precursor farnesyl diphosphate (FDP) is catalyzed by a (+) … mercedes maybach second hand