WebTitration Protocol: To a white opaque 384-well Optiplate: 1- Add 5 μL biotinylated-GST dilutions (from lowest to highest concentration). 2- Add 10 μL of 1x buffer 3- Add 5 μL of Anti-GST (Glutathione S-Transferase) Acceptor beads. Incubate in the dark at room temperature for 30 minutes. 4- Add 5 μL of Streptavidin Donor beads. WebGlutathione S-transferases (GSTs), previously known as ligandins, are a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of …
GST (Glutathione S Transferase) Detection Kit - PerkinElmer
WebThere seems to be an inadvertent problem while communicating with GST System. This may be on account of one of the following: Server is unable to fetch data from backend application. The data from your internet browser (may be Internet Explorer, Chrome, Firefox, Safari etc.) is corrupted during transmission to GST System. WebMoreover, since GST protein might undergo dimerization, 36 parallel titrations were performed using free SH3 m-cort domain and either P2 or S1 peptide (Figure 1, panel C and D). The K d values ... pottawattamie milwaukee
Near-UV CD titration of the HPK1-derived peptide P2 versus ...
WebAug 29, 2024 · 3.13: Titrations. A titration is a volumetric technique in which a solution of one reactant (the titrant) is added to a solution of a second reactant (the "analyte") until … WebWhere students gain the knowledge and skills to become future STEM innovators and leaders. The Governor’s School is a selective magnet program that serves high achieving juniors and seniors from seven … WebBiotinylated-GST at 0.5 µM in 25mM Hepes, 100mM NaCl, 0.05% Proclin-300, 0.05% Tween 20, pH 7.4 1 x 1.5 mL (6760305) Product Information Product Information: The biotinylated-GST probe is designed to be used with AlphaScreen® GST detection kit (6760603). Due to the length of the linker used for the biotinylation, the potteens