Nettet1. Hoechst 33342 Staining solution (HO) 2. Hoechst 33342 1.0 mg/ml in dH2O 3. DiOC5 (Molecular Probes) 1.0mg/ml in DMSO . Staining . 1. HO is added to cells in culture medium at from 1.05.0 - ug/ml. 2. Cells are incubated in HO at 37 degrees C for 30-60 minutes. 3. Cells are analysed without washing while in the media containing the HO. Tips Nettet1. jan. 2011 · One advantage of Hoechst 33342 is that it is membrane permeant and, thus, can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the …
Hoechst Stain 23491-45-4 - Sigma-Aldrich
NettetHoechst 33342 Staining Protocol λ Ex /λ Em (with DNA) = 350/461 nm MW: 561.93 (in H 2 O); 615.98 (trihydrochloride trihydrate) Molecular formula: C 27 H 31 Cl 3 N 6 O CAS … NettetHoechst 33258 solution 1 mg/mL in H2O, ≥98.0% (HPLC) Synonym (s): bisBenzimide H 33258 solution Empirical Formula (Hill Notation): C25H24N6O · 3HCl CAS Number: 23491-45-4 Molecular Weight: 533.88 PubChem Substance ID: 329770334 NACRES: NA.32 Pricing and availability is not currently available. Properties Quality Level 100 … couchside computer stand
Hoechst or DAPI for nuclear staining? - Biology Stack Exchange
NettetPreparing Hoechst dye stock solution. 1. Prepare the Hoechst dye stock solution by dissolving the contents of one vial (100 mg) in 10 mL of deionized water (diH 2 O) to create a 10 mg/mL (16.23 mM) solution. Note: Hoechst dye has poor solubility in … Nettet26. nov. 2012 · The Hoechst 33342 dye is similar to DAPI in that both are UV-excited, minor groove-binding, and emit signals proportional to total DNA content. Both are … NettetThe following table contains a protocol with a simple regressive stain that provides a nice balance of nuclear and cytoplasmic stains. This protocol is designed with a mild acid … couch shops in rustenburg