Webbcloned into blunted HindIII(5=)-BamHI(3=) sites of expression vector pHM6 (Invitrogen, Carlsbad, CA), while the 988 bp rat mas PCR fragment was cloned into HindIII (5=)-EcoRI (3=) sites of pHM6 after digestion with HindIII and EcoRI. Chemicals. Mas agonist (AR234960) and inverse agonists (AR244555 and AR305352) were synthesized at Arena … WebbWe identified p90 ribosomal protein S6 kinases (RSKs; also known as MAPK-activated protein kinases, MAPKAP-K1/K2) as the kinase necessary for Thr 198 phosphorylation. RSK-dependent phosphorylation induced 14-3-3 binding and cytoplasmic localization of p27 Kip1 through a Thr 198 phosphorylation-dependent mechanism.
expression vector pHM6
http://www.protocol-online.org/biology-forums/posts/5295.html Webba pc5FLAG vector was generated by polymerase chain reaction (PCR) with an I.M.A.G.E clone (clone ID 6169151; Open Biosystems, Huntsville, AL, USA) as the template. The p60 c-src cDNA in a pHM6 vector was generated by PCR with an I.M.A.G.E clone (clone ID 4548399; Open Biosystems) as the template. Deletion mutants of TUSC4 were … bmw2002 プラグコード
pHM6 - 57117 ATCC
Webb1 nov. 2003 · A mutation in the POU4F3 gene (BRN-3.1, BRN3C) is responsible for DFNA15 (MIM 602459), autosomal-dominant nonsyndromic hearing loss. POU4F3 is a member of the POU family of transcription factors and is essential for inner-ear hair cell maintenance. Webb1 apr. 2015 · Opossum kidney (OK) cells were transfected with pHM6 empty vector (control) or a pHM6-DNPEP construct (DNPEP) and harvested 48 h later. Measurements of monomeric (G) and total cellular actin was performed by DNase I inhibition assay to determine the G/F-actin ratios, the ratio of F-actin content to total cellular protein, and … Webb11 juni 2024 · Read "10.1016/S1357-2725(01)00006-1" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. 図面 スケールバー 描き方