WebA database of monomeric protein crystal structures was used to teach computer-simulated neural networks rules for predicting surface exposure from local sequence. These trained … WebNov 14, 2024 · 3.1 Biotinylation of Cell Surface Proteins. 1. Seed cells growing as monolayers at a density of 5 × 10 4 cells/cm 2; cells growing in suspension should be seeded at 2–5 × 10 5 cells/mL. Depending on the cell type, cells seeded as above should reach confluence within 3–5 days from seeding ( see Note 1 ). 2.

Surface protein imputation from single cell …

WebSep 23, 2024 · Using a limited set of cell surface protein measurements, researchers can therefore impute the presence of a large set of proteins on the cells in their sample. The method consists of two steps. First, the researchers stain all the cells in an experiment with antibodies to a small number of proteins. WebCell surface proteins are major targets of biomedical research due to their utility as cellular markers and their extracellular accessibility for pharmacological intervention. However, information about the cell surface protein repertoire (the surfaceome) of individual cells is only sparsely available. set the secret_key environment variable

Protein surface representation and analysis by dimension …

WebThe only problem with the 2 suggestions you have is: 1) For flow cytometry, you must know the composition of the protein on the surface in order to attach the fluorescent molecule … WebCharacterizing protein–protein interactions through methods such as co-immunoprecipitation (co-IP), pull-down assays, crosslinking, label transfer, and … WebApr 9, 2008 · Analysis performed on small data sets have shown [] that geometrical properties of the protein surface influence protein-protein interactions.For the analysis of larger data sets (e.g. the non-redundant set of PDB structures), tools that can process a wider range of structural information descriptors are necessary. set the seal on meaning